| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100B; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human S100B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BCB-19 is a rabbit monoclonal antibody that recognizes protein S100-B (gene S100B) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 10.7 kDa, and the supplier reports an observed band at about 11 kDa.
S100B is a small calcium- and zinc-binding protein that is highly abundant in astrocytes and other glial cells. Inside cells it regulates target proteins such as kinases, and when secreted it can act as a neurotrophic factor or, at higher levels, signal through RAGE, so it is widely used as a glial and brain injury marker in research.
| Target | Protein S100-B (gene S100B; UniProt P04271, human) |
|---|---|
| Clone | BCB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 10.7 kDa |
| Observed MW | 11 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): rat brain tissue, rat C6 cells, mouse brain tissue, A375 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): mouse pancreas tissue, rat cerebral cortex tissue, mouse hippocampus tissue, human testis cancer tissue, human glioblastoma tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): rat C6 cells.
Immunogen
Synthetic peptide based on the sequence of human S100B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes protein S100-B as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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