| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sphingosine 1-phosphate receptor 3; S1P receptor 3; S1P3; Endothelial differentiation G protein-coupled receptor 3; Sphingosine 1-phosphate receptor Edg-3; S1P receptor Edg-3; S1PR3; C9orf108; C9orf47; EDG3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human S1PR3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24S03 is a rabbit monoclonal antibody that recognizes sphingosine 1-phosphate receptor 3 (gene S1PR3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 42.3 kDa, and the supplier reports an observed band at about 42 kDa.
S1PR3 (also known as EDG3) is a G protein-coupled receptor for sphingosine 1-phosphate that can couple to several G protein classes. It influences cell survival, proliferation and migration, and it is studied in vascular biology, inflammation and fibrosis.
| Target | Sphingosine 1-phosphate receptor 3 (gene S1PR3; UniProt Q99500, human) |
|---|---|
| Clone | 24S03 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.3 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human A549 cells, human SiHa cells, rat C6 cells, rat NRK cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human S1PR3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sphingosine 1-phosphate receptor 3 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Xiaohui et al. (2026) Decreasing S1PR3 Alleviated Renal Tubular Lipid Deposition in Diabetic Nephropathy via AMPK/PPAR-γ/GPAT3 Pathway. Applied Biochemistry and Biotechnology. 10.1007/s12010-026-05640-x
