| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cohesin subunit SA-2; SCC3 homolog 2; Stromal antigen 2; STAG2; SA2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SA2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADEG-19 is a rabbit monoclonal antibody that recognizes cohesin subunit SA-2 (gene STAG2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 141.3 kDa, and the supplier reports an observed band at about 141 kDa.
STAG2 (SA2) is a subunit of the cohesin complex, which encircles sister chromatids to keep them paired until their separation at anaphase. Cohesin also shapes 3D genome structure and gene expression, and STAG2 is frequently mutated in several cancers, including bladder cancer and myeloid neoplasms.
| Target | Cohesin subunit SA-2 (gene STAG2; UniProt Q8N3U4, human) |
|---|---|
| Clone | ADEG-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 141.3 kDa |
| Observed MW | 141 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:200 |
Samples with a confirmed band (WB): K562 cells, Raw 2647 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SA2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cohesin subunit SA-2 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
