| Field | Specification |
|---|---|
| Target | |
| Alternative names | SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; SAE1; AOS1; SUA1; UBLE1A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SAE1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19S08 is a rabbit monoclonal antibody that recognizes SUMO-activating enzyme subunit 1 (gene SAE1) in human and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 38.5 kDa, and the supplier reports an observed band at about 38 kDa.
SAE1 (also called AOS1) is the smaller subunit of the SUMO-activating enzyme, working as a heterodimer with UBA2 (SAE2). This E1 enzyme uses ATP to activate SUMO proteins and passes them on to the conjugating enzyme UBC9, so it controls the first step of protein SUMOylation.
| Target | SUMO-activating enzyme subunit 1 (gene SAE1; UniProt Q9UBE0, human) |
|---|---|
| Clone | 19S08 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.5 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human 293T cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human SAE1.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places SUMO-activating enzyme subunit 1 in the nucleus. Tissue expression noted by UniProt (human): Expression level increases during S phase and drops in G2 phase (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
