| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc finger FYVE domain-containing protein 9; Mothers against decapentaplegic homolog-interacting protein; Madh-interacting protein; Novel serine protease; NSP; Receptor activation anchor; hSARA; Smad anchor for receptor activation; ZFYVE9; MADHIP; SARA; SMADIP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SARA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28Z89 is a rabbit monoclonal antibody that recognizes zinc finger FYVE domain-containing protein 9 (gene ZFYVE9) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 156.4 kDa, and the supplier reports an observed band at about 180 kDa.
ZFYVE9, also called SARA (SMAD anchor for receptor activation), is an early endosome protein whose FYVE domain binds phosphatidylinositol 3-phosphate. It presents SMAD2 and SMAD3 to the activated TGF-β receptor so they can be phosphorylated, making it a key adaptor in TGF-β signaling.
| Target | Zinc finger FYVE domain-containing protein 9 (gene ZFYVE9; UniProt O95405, human) |
|---|---|
| Clone | 28Z89 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 156.4 kDa |
| Observed MW | 180 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): A673 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SARA.
Reactivity Notes
The supplier lists reactivity with human. UniProt places zinc finger FYVE domain-containing protein 9 in the cytoplasm and early endosome membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
