Anti-SARS-CoV-2 ORF3a Antibody

SKU:BHA21003167
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-3a antibody (Rabbit polyclonal, Rabbit IgG). Validated for ELISA. Reactivity: Human. Recognizes ORF3a protein. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target 3a
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, IHC, Flow Cytometry, ELISA
Options selector
Catalog no. Size Conjugation
A33994 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial / Carrier Free, 100 ug/vial / Unconjugated: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.; Form: Lyophilized; Applications: ELISA; Application details: ELISA, 0.001-0.1μg/ml, Human; Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial / APC, 100 ug/vial / Biotin, 100 ug/vial / Cy3, 100 ug/vial / FITC, 100 ug/vial / Fluoro488, 100 ug/vial / Fluoro550, 100 ug/vial / Fluoro594, 100 ug/vial / Fluoro647, 100 ug/vial / PE: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.; Form: Liquid; Applications: Flow Cytometry, WB,IHC,ELISA; Application details: Flow Cytometry, 1-3μg/1x106 cells; Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing., At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial / HRP: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.; Form: Liquid; Applications: WB,IHC,ELISA; Application details: Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: varies by selected option; please contact us for current fulfillment timing.
  • Storage: varies by selected option; see option details above.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target 3a
Alternative names ORF3a protein; ORF3a; Accessory protein 3a; Protein 3a; Protein U274; Protein X1
UniProt # P0DTC3
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • ELISA
Immunogen MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASggggFQSASKIITLKKRWQLALSKGVHFVCNLggggQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Cytoplasm .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) A33994
Main SKU BHA21003167
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody detects ORF3a protein in human samples and is validated for ELISA.

Target ORF3a protein (UniProt P0DTC3, Severe acute respiratory syndrome coronavirus 2)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

ELISA 0.001–0.1 µg/mL (Human)

Immunogen

MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASggggFQSASKIITLKKRWQLALSKGVHFVCNLggggQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL.

Reactivity Notes

The supplier lists reactivity with human. UniProt describes ORF3a protein as secreted and also located in the virion, host cell membrane and host endoplasmic reticulum membrane.

Safety

For research use only; not for diagnostic or therapeutic procedures. Handle with standard laboratory precautions.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: ELISA 0.001–0.1 µg/mL (Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASggggFQSASKIITLKKRWQLALSKGVHFVCNLggggQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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