| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stearoyl-CoA desaturase; hSCD1; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Fatty acid desaturase; SCD; FADS5; SCD1; SCDOS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SCD recombinant protein (Position: R26-S358). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects stearoyl-CoA desaturase (gene SCD) in human samples and is validated for Western blot, ICC/IF, flow cytometry, immunoprecipitation and ELISA.
Stearoyl-CoA desaturase (SCD, also SCD1) is an ER membrane enzyme that introduces a cis double bond into saturated fatty acyl-CoAs, mainly converting palmitoyl- and stearoyl-CoA to palmitoleate and oleate. As the rate-limiting step in monounsaturated fatty acid synthesis, it is widely studied in lipogenesis, obesity, insulin resistance and cancer metabolism.
| Target | Stearoyl-CoA desaturase (gene SCD; UniProt O00767, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human SCD fragment (Arg26–Ser358), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places stearoyl-CoA desaturase in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Detected in fetal liver, lung and brain.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
