| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kit ligand;Mast cell growth factor;MGF;Stem cell factor;SCF;c-Kit ligand;Soluble KIT ligand;sKITLG;KITLG;MGF, SCF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SCF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FAG-11 is a rabbit monoclonal antibody that recognizes kit ligand (gene KITLG) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 30.9 kDa, and the supplier reports an observed band at about 18 kDa.
Kit ligand (KITLG, also called stem cell factor or SCF) is a cytokine that activates the KIT receptor tyrosine kinase. It exists in membrane-bound and soluble forms and supports the survival and proliferation of hematopoietic stem and progenitor cells, mast cells, melanocytes and germ cells. SCF is widely used in stem cell culture and studied in hematopoiesis, pigmentation and fertility.
| Target | Kit ligand (gene KITLG; UniProt P21583, human) |
|---|---|
| Clone | FAG-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.9 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with confirmed staining (IHC): human stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human SCF.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
