| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium channel protein type 1 subunit alpha;Sodium channel protein brain I subunit alpha;Sodium channel protein type I subunit alpha;Voltage-gated sodium channel subunit alpha Nav1.1;SCN1A;NAC1, SCN1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Scn1a, identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sodium channel protein type 1 subunit alpha (gene SCN1A) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 229.0 kDa, and the supplier reports an observed band at about 250 kDa.
SCN1A encodes the channel-forming α subunit of Nav1.1, one of the voltage-gated sodium channels that drive the rising phase of action potentials. Nav1.1 is especially important in inhibitory interneurons in the brain, and SCN1A mutations are a major cause of genetic epilepsies such as Dravet syndrome.
| Target | Sodium channel protein type 1 subunit alpha (gene SCN1A; UniProt P35498, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 229.0 kDa |
| Observed MW | 250 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human scn1a. The peptide sequence is conserved in rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium channel protein type 1 subunit alpha in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chen Ajuan et al. (2022) mTORC1 induces plasma membrane depolarization and promotes preosteoblast senescence by regulating the sodium channel Scn1a. Bone Research. 10.1038/s41413-022-00204-1
Shi Hao-Song et al. (2019) Ca2+-dependent recruitment of voltage-gated sodium channels underlies bilirubin-induced overexcitation and neurotoxicity. Cell Death & Disease. 10.1038/s41419-019-1979-1
