| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium channel protein type 2 subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.2; Scn2a; Scn2a1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Scn2a, which shares 82.4% and 94.1% amino acid (aa) sequence identity with human and rat Scn2a, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sodium channel protein type 2 subunit alpha (gene Scn2a) in mouse and rat samples and is validated for Western blot.
SCN2A encodes the α subunit of Nav1.2, a voltage-gated sodium channel expressed mainly in excitatory neurons of the central nervous system. It contributes to action potential initiation and propagation, and SCN2A variants are associated with epilepsy and neurodevelopmental disorders including autism.
| Target | Sodium channel protein type 2 subunit alpha (gene Scn2a; UniProt B1AWN6, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.005 mg NaN3 |
| Observed MW | 320 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of mouse scn2a. Sequence identity with the human and rat orthologs: 82.4% and 94.1%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places sodium channel protein type 2 subunit alpha in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed in brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
