Anti-SEC24D Rabbit Monoclonal Antibody

SKU:BHA21010532
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Overview
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Anti-SEC24D antibody (Rabbit monoclonal, clone 30S42, IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes protein transport protein Sec24D. Observed band in Western blot: about 113 kDa.
Target SEC24D
clone number 30S42
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M06985 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB
      • Application details: WB 1:500-2000
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target SEC24D
Alternative names Protein transport protein Sec24D; SEC24-related protein D; SEC24D; KIAA0755
Gene ID 9871
UniProt # O94855
Host Rabbit
Clone 30S42
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human SEC24D
Molecular weight 113.0 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M06985
Main SKU BHA21010532
Rabbit Monoclonal

Product Overview

Clone 30S42 is a rabbit monoclonal antibody that recognizes protein transport protein Sec24D in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 113.0 kDa, and the supplier reports an observed band at about 113 kDa.

SEC24D is a cargo-binding subunit of the COPII coat that selects proteins for export from the endoplasmic reticulum to the Golgi. It is especially important for secretion of collagen and other matrix proteins, and SEC24D mutations cause a syndromic form of osteogenesis imperfecta.

Target Protein transport protein Sec24D (gene SEC24D; UniProt O94855, human)
Clone 30S42
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 113.0 kDa
Observed MW 113 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): HeLa cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human SEC24D.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places protein transport protein Sec24D in the endoplasmic reticulum membrane and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed, with higher amounts in placenta, pancreas, heart and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 113.0 kDa; observed band at about 113 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human SEC24D.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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