| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein transport protein Sec61 subunit beta; SEC61B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SEC61B recombinant protein (Position: R16-S96). Human SEC61B shares 98.8% amino acid (aa) sequence identity with mouse SEC61B. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein transport protein Sec61 subunit beta (gene SEC61B) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
SEC61B is the β subunit of the SEC61 translocon, the protein-conducting channel of the endoplasmic reticulum membrane. The translocon binds ribosomes and lets newly made secretory proteins cross the membrane or insert as transmembrane proteins, and SEC61B is commonly used as an ER marker.
| Target | Protein transport protein Sec61 subunit beta (gene SEC61B; UniProt P60468, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human SEC61B fragment (Arg16–Ser96), expressed in E. coli. Sequence identity with the mouse ortholog: 98.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein transport protein Sec61 subunit beta in the endoplasmic reticulum membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
