| Field | Specification |
|---|---|
| Target | |
| Alternative names | Selenocysteine insertion sequence-binding protein 2; SECIS-binding protein 2; SECISBP2; SBP2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SECISBP2 recombinant protein (Position: E12-L854). Human SECISBP2 shares 71.7% amino acid (aa) sequence identity with rat SECISBP2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects selenocysteine insertion sequence-binding protein 2 (gene SECISBP2) in human samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 95.5 kDa, and the supplier reports an observed band at about 105 kDa.
SECISBP2 (SBP2) is an RNA-binding protein that recognizes the SECIS element in the 3′ untranslated region of selenoprotein mRNAs. By recruiting the elongation factor EEFSEC and the ribosome, it lets a UGA codon be read as selenocysteine instead of stop, making it essential for selenoprotein synthesis and thyroid hormone metabolism.
| Target | Selenocysteine insertion sequence-binding protein 2 (gene SECISBP2; UniProt Q96T21, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 95.5 kDa |
| Observed MW | 105 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Recombinant human SECISBP2 fragment (Glu12–Leu854), expressed in E. coli. Sequence identity with the rat ortholog: 71.7%.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed at high levels in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
