| Field | Specification |
|---|---|
| Target | |
| Alternative names | Securin; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG; PTTG1; EAP1; PTTG; TUTR1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADFH-16 is a rabbit monoclonal antibody that recognizes securin (gene PTTG1) in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 22.0 kDa, and the supplier reports an observed band at about 26 kDa.
Securin (PTTG1) binds and inhibits separase until the metaphase-to-anaphase transition. When the anaphase-promoting complex tags securin for destruction, separase is freed to cleave cohesin so sister chromatids can separate, making securin a key protein in studies of chromosome segregation; it is also overexpressed in pituitary and other tumors.
| Target | Securin (gene PTTG1; UniProt O95997, human) |
|---|---|
| Clone | ADFH-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.0 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
| Flow cytometry | 1:50 |
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair.
Reactivity Notes
The supplier lists reactivity with human. UniProt places securin in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed at low level in most tissues, except in adult testis, where it is highly expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Mengchen et al. (2016) APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica. Scientific Reports. 10.1038/srep39047
Peng Tan et al. (2026) Discovery of furan-trimethoxyphenyl hybrids as novel colchicine-binding site inhibitors that induce mitotic catastrophe with potent anti-acute myeloid leukemia effect. Bioorganic Chemistry. 10.1016/j.bioorg.2026.110109
Xiao-Ming Liu et al. (2021) Checkpoint kinases are required for oocyte meiotic progression by the maintenance of normal spindle structure and chromosome condensation. Experimental Cell Research. 10.1016/j.yexcr.2021.112657
Zhang Enjiao et al. (2014) Pituitary tumor-transforming gene 1 (PTTG1) is overexpressed in oral squamous cell carcinoma (OSCC) and promotes migration, invasion and epithelial–mesenchymal transition (EMT) in SCC15 cells. Tumor Biology. 10.1007/s13277-014-2143-2
