| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sentrin-specific protease 1; Sentrin/SUMO-specific protease SENP1; SENP1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SENP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19S57 is a rabbit monoclonal antibody that recognizes sentrin-specific protease 1 (gene SENP1) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 73.5 kDa, and the supplier reports an observed band at about 73 kDa.
SENP1 is a SUMO-specific cysteine protease with two jobs: it trims SUMO1, SUMO2 and SUMO3 precursors into their mature forms, and it removes SUMO from modified proteins. By reversing SUMOylation it controls the activity and localization of many nuclear proteins, including transcription factors, and is studied in gene regulation, hypoxia signaling and cancer biology.
| Target | Sentrin-specific protease 1 (gene SENP1; UniProt Q9P0U3, human) |
|---|---|
| Clone | 19S57 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 73.5 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:30 |
Samples with a confirmed band (WB): U87-MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SENP1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sentrin-specific protease 1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
