| Field | Specification |
|---|---|
| Target | |
| Alternative names | Septin-2; Neural precursor cell expressed developmentally down-regulated protein 5; NEDD-5; SEPTIN2; DIFF6; KIAA0158; NEDD5; SEPT2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Septin 2 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18S62 is a rabbit monoclonal antibody that recognizes septin-2 (gene SEPTIN2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 41.5 kDa, and the supplier reports an observed band at about 41 kDa.
Septin-2 (SEPTIN2) is a widely expressed septin GTPase that forms filaments with other septins. It helps organize the actin cytoskeleton, supports cytokinesis and epithelial polarity, and contributes to the sperm annulus, making it a common subject in studies of cell division, cell shape and cytoskeletal organization.
| Target | Septin-2 (gene SEPTIN2; UniProt Q15019, human) |
|---|---|
| Clone | 18S62 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.5 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human septin 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places septin-2 in the cytoplasm, chromosome, cleavage furrow and midbody. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
