| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Lymphocyte antigen 6A-2/6E-1;Ly-6A.2/Ly-6E.1;Stem cell antigen 1;SCA-1;T-cell-activating protein;TAP;Ly6a;Ly6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Septin 8/Septin8, which shares 95.7% and 100% amino acid (aa) sequence identity with human and rat SEPTIN8, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Septin 8/Septin8 Antibody Picoband® is an antibody reagent for detection of Septin8 (Lymphocyte antigen 6A-2/6E-1). Researchers commonly use anti-Septin8 antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, Flow, ELISA).
Boster Bio Anti-Septin 8/Septin8 Antibody Picoband® catalog # A30760-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: Septin8 — Lymphocyte antigen 6A-2/6E-1 (Lymphocyte antigen 6A-2/6E-1). Alternative names: Lymphocyte antigen 6A-2/6E-1;Ly-6A.2/Ly-6E.1;Stem cell antigen 1;SCA-1;T-cell-activating protein;TAP;Ly6a;Ly6;
- Antibody format: Polyclonal; Rabbit IgG
- Species context: Host: Rabbit, Reactivity: Mouse,Rat
- Purification: Immunogen affinity purified.
- Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Septin 8/Septin8, which shares 95.7% and 100% amino acid (aa) sequence identity with human and rat SEPTIN8, respectively.
- Molecular weight context: observed 56 kDa, calculated 14377 MW (reported)
- Provided application(s): WB, IHC, Flow, ELISA
These attributes help contextualize how the antibody is commonly selected (host/clonality/isotype/label) and how signals are interpreted across sample types and assay formats.
Biological background
Function: T-cell activation.
Cellular localization: Cell membrane; Lipid-anchor, GPI-anchor.
Tissue details: Widely expressed. .
Background: Predicted to enable GTPase activity; SNARE binding activity; and molecular adaptor activity. Predicted to be involved in several processes, including regulation of SNARE complex assembly; regulation of intracellular protein transport; and regulation of protein stability. Located in myelin sheath. Part of septin complex. Is expressed in dermis; spinal cord ventricular layer; turbinate bone primordium; and ventral grey horn. Orthologous to human Septin8 (septin 8).
Cross reactivity: No cross-reactivity with other proteins.
Research relevance and current trends
- Quantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.
- Isoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.
- Context-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.
Common research applications
- Western blot (WB): compare relative target abundance and apparent size shifts (e.g., isoforms/PTMs) across conditions.
- Immunohistochemistry (IHC): assess distribution across tissue compartments and compare staining patterns between groups.
- Flow cytometry: quantify target-positive populations and compare shifts after stimulation or differentiation.
Across these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.
Notes for experimental interpretation
- Apparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.
- Species reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.
- Control concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO/KD, peptide competition, or independent assays) to support interpretation.
For antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
