| Field | Specification |
|---|---|
| Target | |
| Alternative names | SERPINE1 mRNA-binding protein 1; PAI1 RNA-binding protein 1; PAI-RBP1; Plasminogen activator inhibitor 1 RNA-binding protein; SERBP1; PAIRBP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SERBP1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects SERPINE1 mRNA-binding protein 1 (gene SERBP1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 45.0 kDa; the supplier reports observed bands at 55 kDa(human), 50 kDa(mouse, rat).
SERBP1 (SERPINE1 mRNA-binding protein 1, also called PAI-RBP1) is an RNA- and ribosome-binding protein. It associates with eEF2 on idle ribosomes to hold them in a stable, inactive hibernation state and may also regulate mRNA stability, including that of the PAI-1 transcript.
| Target | SERPINE1 mRNA-binding protein 1 (gene SERBP1; UniProt Q8NC51, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 45.0 kDa |
| Observed MW | 55 kDa(human), 50 kDa(mouse, rat) |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human LNCAP cells, human K562 cells, human Daudi cells, human HEL cells, rat kidney tissue, mouse skeletal muscle tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): ANA-1 cells, HEL cells, RH35 cells.
Immunogen
Synthetic peptide from the C-terminal region of human SERBP1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places SERPINE1 mRNA-binding protein 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li-Xia Hu et al. (2023) Alterations in the endometrium of rats, rabbits, and Macaca mulatta that received an implantation of copper/low-density polyethylene nanocomposite. International Journal of Nanomedicine. 10.2147/IJN.S56756
Wang Xiaohui et al. (2026) Decreasing S1PR3 Alleviated Renal Tubular Lipid Deposition in Diabetic Nephropathy via AMPK/PPAR-γ/GPAT3 Pathway. Applied Biochemistry and Biotechnology. 10.1007/s12010-026-05640-x
Yang Chen et al. (2015) Suppression of plasminogen activator inhibitor-1 by inhaled nitric oxide attenuates the adverse effects of hyperoxia in a rat model of acute lung injury. Thrombosis Research. 10.1016/j.thromres.2015.04.018
