| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca(2+)-ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATP2A1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SERCA1 ATPase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24A90 is a rabbit monoclonal antibody that recognizes sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (gene ATP2A1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 110.3 kDa, and the supplier reports an observed band at about 110 kDa.
ATP2A1 (SERCA1) is the calcium pump of fast-twitch skeletal muscle. It uses ATP to pump Ca2+ from the cytosol back into the sarcoplasmic reticulum, allowing muscle relaxation after contraction, and its deficiency causes Brody myopathy.
| Target | Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (gene ATP2A1; UniProt O14983, human) |
|---|---|
| Clone | 24A90 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 110.3 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human fetal muscle.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SERCA1 ATPase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sarcoplasmic/endoplasmic reticulum calcium ATPase 1 in the endoplasmic reticulum membrane and sarcoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Skeletal muscle, fast twitch muscle (type II) fibers. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
