| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sarcoplasmic/endoplasmic reticulum calcium ATPase 3;SERCA3;SR Ca (2+)-ATPase 3;3.6.3.8;Calcium pump 3;ATP2A3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ATP2A3, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ATP2A3 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-SERCA3 ATPase/ATP2A3 Antibody catalog # RP1055. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human ATP2A3, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.
- Molecular weight context: reported MW: 114 kDa; calculated MW: 113977 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3; Sarcoplasmic/endoplasmic reticulum calcium ATPase 3. Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, also known as SERCA3, is anenzyme that in humans is encoded by the ATP2A3 gene. It is mapped to 17p13.2. This gene encodes one of the SERCA Ca2+-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of cells. ATP2A3 expression was originally described as non-muscular, but was recently observed in cardiomyocyte. What’s more, the expression of ATP2A3 was significantly reduced or lost in colon carcinomas compared with normal colonic epithelial cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Functional note: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. . Reported localization: Nucleus membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Sarcoplasmic reticulum membrane ; Multi-pass membrane protein . Expression/tissue context: Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Expressed in cell lineages of hematopoietic, epithelial, or embryonic origin and also expressed in several cancer cell lines. .
Research relevance and current trends
- ATPases: Researchers commonly examine how ATP2A3 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3) relates to this theme using model systems and orthogonal readouts.
- Calcium Signaling: Researchers commonly examine how ATP2A3 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how ATP2A3 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ATP2A3 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ATP2A3 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
