| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | B-cell CLL/lymphoma 9-like protein; B-cell lymphoma 9-like protein; BCL9-like protein; Protein BCL9-2; BCL9L; DLNB11 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse SERINC3 recombinant protein (Position: E59-Y404). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SERINC3 Antibody Picoband® is an antibody for Serinc3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Serinc3 (BCL9 like); UniProt: Q9QZI9
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 53 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-SERINC3 Antibody Picoband® catalog # A05936-2.
Biological background
Biological context: Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1.
Expression and localization notes: cellular localization: Nucleus., tissue context: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor..
Common research applications
- Western blotting (WB): Compare Serinc3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Serinc3 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify Serinc3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Serine incorporator 3 is a protein that in humans is encoded by the SERINC3 gene. Also found on the membranes of the Golgi apparatus within cells, TDE1 is highly expressed in neuronal populations but is also found in thymus, kidney, liver and testis. Expression levels of TDE1 in tumors can be as much as tenfold the amount found in normal tissue of the same type. This increased expression implicates TDE1 as being involved in the cellular transformation from normal to malignant tissue. It is believed TDE1 contributes to oncogenesis by partially protecting cells from serum starvation and etoposide-induced apoptosis. The mechanism through which TDE1 protects cells is poorly understood, but may involve aberrant methylation of TDE1 complexes.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.
- Research category: Cell Biology,Cell Cycle,Cell Differentiation,G Protein Signaling,Regulators,Signal Transduction,Signaling Pathway,Small G Proteins
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
