Anti-Serotonin transporter/SLC6A4 Antibody Picoband®

Overview

This antibody is intended for detection of SLC6A4 (Sodium-dependent serotonin transporter) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-Serotonin transporter/SLC6A4 Antibody Picoband® catalog # PB9438. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A4, different from the related mouse and rat sequences by six amino acids.
• Molecular weight context: reported MW: 70-90 kDa; calculated MW: 70325 MW
• Reactivity: Human,Mouse,Rat
• Applications: IF, IHC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Sodium-dependent serotonin transporter; Sodium-dependent serotonin transporter. SLC6A4 (Solute carrier family 6, member 4), also known as SERT (serotonin transporter), is a monoamine transporter protein. The SLC6A4 gene spans 31 kb and contains 14 exons. This protein integral membrane protein transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is the target of many antidepressant medications, including those of the SSRI class. It is a member of the sodium: neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma. Functional note: Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. . Reported localization: Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. Expression/tissue context: Expressed in platelets (at protein level). .

Research relevance and current trends

• Drug Metabolism: Researchers commonly examine how SLC6A4 (Sodium-dependent serotonin transporter) relates to this theme using model systems and orthogonal readouts.
• Metabolic Signaling Pathways: Researchers commonly examine how SLC6A4 (Sodium-dependent serotonin transporter) relates to this theme using model systems and orthogonal readouts.
• Metabolism: Researchers commonly examine how SLC6A4 (Sodium-dependent serotonin transporter) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative SLC6A4 (Sodium-dependent serotonin transporter) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of SLC6A4 (Sodium-dependent serotonin transporter) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.