| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium-dependent serotonin transporter;5HT transporter;5HTT;Solute carrier family 6 member 4;SLC6A4;HTT, SERT; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A4, different from the related mouse and rat sequences by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody recognizes sodium-dependent serotonin transporter (gene SLC6A4) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 70.3 kDa, and the supplier reports an observed band at about 70–90 kDa.
SLC6A4 encodes the serotonin transporter (SERT), which clears serotonin from the extracellular space by sodium-coupled uptake into the cell. It sets the duration of serotonergic signaling in the brain and gut, is the target of selective serotonin reuptake inhibitors, and is also the route by which platelets accumulate serotonin.
| Target | Sodium-dependent serotonin transporter (gene SLC6A4; UniProt P31645, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 70.3 kDa |
| Observed MW | 70–90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): human SH-SY5Y cells, human U2OS cells, human SW620 cells, human HeLa cells, human 293T cells, human MCF-7 cells, human A549 cells, mouse NIH/3T3 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells, human HepG2 cells, human Caco-2 cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): mouse brain tissue, rat brain tissue.
Immunogen
Synthetic peptide from the N-terminal region of human SLC6A4. It differs from the mouse and rat sequences at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium-dependent serotonin transporter in the cell membrane, endomembrane system, endosome membrane and synapse. Tissue expression noted by UniProt (human): Expressed in platelets (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhengjiang Liu et al. (2018) Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated Protein-43 in Rats. Biomed Research International. 10.1155/2018/8697913
Ming Liu et al. (2012) Fluoxetine Attenuates Chronic Methamphetamine-induced Pulmonary Arterial Remodelling: Possible Involvement of Serotonin Transporter and Serotonin 1B Receptor. Basic & Clinical Pharmacology & Toxicology. 10.1111/j.1742-7843.2012.00933.x
Tongjun Ma et al. (2023) Regulatory Effect of Electroacupuncture on Hypothalamic Serotonin and its Receptor in Rats with Cerebral Ischemia. Current Neurovascular Research. 10.2174/1567202620666230612110156
Zheng-Jiang Liu et al. (2019) Effect of sepsis on the action potential and cardiac serotonin response in rats. Experimental and Therapeutic Medicine. 10.3892/etm.2019.7810
