| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium-dependent serotonin transporter;5HT transporter;5HTT;Solute carrier family 6 member 4;Slc6a4; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of rat SLC6A4, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sodium-dependent serotonin transporter (gene Slc6a4) in rat samples and is validated for Western blot and IHC. The predicted molecular weight is 70.2 kDa, and the supplier reports an observed band at about 70 kDa.
SLC6A4 encodes the serotonin transporter (SERT), which clears serotonin from the extracellular space by sodium-coupled uptake into the cell. It sets the duration of serotonergic signaling in the brain and gut, is the target of selective serotonin reuptake inhibitors, and is also the route by which platelets accumulate serotonin.
| Target | Sodium-dependent serotonin transporter (gene Slc6a4; UniProt P31652, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 70.2 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Rat) |
Samples with a confirmed band (WB): rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of rat SLC6A4. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places sodium-dependent serotonin transporter in the cell membrane, endomembrane system, endosome membrane and synapse. Tissue expression noted by UniProt (rat): Expressed in the intestinal crypt epithelial cells and myenteric neurons of the small intestine (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Harraz Maged M. et al. (2021) Cocaine-induced locomotor stimulation involves autophagic degradation of the dopamine transporter. Molecular Psychiatry. 10.1038/s41380-020-00978-y
Shao-Ping Zhu et al. (2009) CONTINUOUS FLUOXETINE ADMINISTRATION PREVENTS RECURRENCE OF PULMONARY ARTERIAL HYPERTENSION AND PROLONGS SURVIVAL IN RATS. Clinical and Experimental Pharmacology and Physiology. 10.1111/j.1440-1681.2009.05181.x
