| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Plasminogen activator inhibitor 2;PAI-2;Monocyte Arg-serpin;Placental plasminogen activator inhibitor;Serpin B2;Urokinase inhibitor;SERPINB2;PAI2, PLANH2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human SerpinB2 recombinant protein (Position: M1-K180). Human SerpinB2 shares 70% and 66% amino acid (aa) sequence identity with mouse and rat SerpinB2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SERPINB2 (Plasminogen activator inhibitor 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-SerpinB2 Antibody Picoband® catalog # PB9355. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human SerpinB2 recombinant protein (Position: M1-K180). Human SerpinB2 shares 70% and 66% amino acid (aa) sequence identity with mouse and rat SerpinB2, respectively. (reported region: M1-K180).
- Molecular weight context: reported MW: 47 kDa; calculated MW: 46596 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Plasminogen activator inhibitor 2; Plasminogen activator inhibitor 2. SERPINB2 is also known as PAI or PAI2. Plasminogen activator inhibitor-2 (placental PAI) is acoagulation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosisduring pregnancy. The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide. Functional note: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell- derived PAI-1. Reported localization: Cytoplasm. Secreted, extracellular space. Expression/tissue context: Plasma. .
Research relevance and current trends
- Immunology: Researchers commonly examine how SERPINB2 (Plasminogen activator inhibitor 2) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how SERPINB2 (Plasminogen activator inhibitor 2) relates to this theme using model systems and orthogonal readouts.
- Macrophage/Inflammation: Researchers commonly examine how SERPINB2 (Plasminogen activator inhibitor 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SERPINB2 (Plasminogen activator inhibitor 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of SERPINB2 (Plasminogen activator inhibitor 2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Family / similarity context: Belongs to the semaphorin family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
