| Field | Specification |
|---|---|
| Target | |
| Alternative names | Antithrombin-III; ATIII; Serpin C1; SERPINC1; AT3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SERPINC1 Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACCA-19 is a rabbit monoclonal antibody that recognizes antithrombin-III (gene SERPINC1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 52.6 kDa, and the supplier reports an observed band at about 53 kDa.
Antithrombin-III (antithrombin, SERPINC1) is the main plasma serpin that controls blood coagulation. It inactivates thrombin and factors IXa, Xa and XIa, and its activity is strongly accelerated by heparin, which is the basis of heparin's anticoagulant effect; inherited antithrombin deficiency increases the risk of venous thrombosis.
| Target | Antithrombin-III (gene SERPINC1; UniProt P01008, human) |
|---|---|
| Clone | ACCA-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.6 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SERPINC1.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes antithrombin-III as a secreted protein. Tissue expression noted by UniProt (human): Found in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
