| Field | Specification |
|---|---|
| Target | |
| Alternative names | Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SF3A3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29S29 is a rabbit monoclonal antibody that recognizes splicing factor 3A subunit 3 (gene SF3A3) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 58.8 kDa, and the supplier reports an observed band at about 59 kDa.
Splicing factor 3A subunit 3 (SF3A3, SF3a60) is a subunit of the SF3a complex of the U2 snRNP. It contributes to early spliceosome assembly and to recognition of the intron branch site in pre-mRNA, and it is studied in pre-mRNA splicing in human systems.
| Target | Splicing factor 3A subunit 3 (gene SF3A3; UniProt Q12874, human) |
|---|---|
| Clone | 29S29 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.8 kDa |
| Observed MW | 59 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SF3A3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places splicing factor 3A subunit 3 in the nucleus speckle and nucleus. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
