Anti-SF3B3 Rabbit Monoclonal Antibody

Overview

This product is an anti-SF3B3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 19S30; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-SF3B3 Rabbit Monoclonal Antibody catalog # M08240-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: SF3B3 (DnaJ homolog subfamily C member 15).
• Antibody format: Monoclonal; clone 19S30; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

SF3B3 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (By similarity). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9. . Reported cellular localization context: Mitochondrion inner membrane ; Single-pass membrane protein . Tissue expression notes (as provided): Expressed at highest levels in heart, followed by liver and kidney. .

Research relevance and current trends

• Research context keywords from the source record include: Atherosclerosis,Cancer,Cardiovascular,Diabetes,Diabetes-associated,Growth Factors/Hormones,Heart Disease,Metabolism,Neurology Process,Neuroscience,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Tyrosine Kinases.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate SF3B3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SF3B3 expression by Western blot in cell or tissue lysates, Detect SF3B3 in FFPE tissue sections by immunohistochemistry, Localize SF3B3 by immunofluorescence/immunocytochemistry in cultured cells, Quantify SF3B3-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 136 kDa; calculated MW: 16383 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 136 kDa
• Cellular localization (provided): Mitochondrion inner membrane ; Single-pass membrane protein .
• Tissue details (provided): Expressed at highest levels in heart, followed by liver and kidney. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.