| Field | Specification |
|---|---|
| Target | |
| Alternative names | Secreted frizzled-related protein 4;sFRP-4;Frizzled protein, human endometrium;FrpHE;SFRP4;FRPHE; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human SFRP4 recombinant protein (Position: A22-K303). Human SFRP4 shares 96.8% and 96.1% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes secreted frizzled-related protein 4 (gene SFRP4) in human samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 39.8 kDa, and the supplier reports an observed band at about 49 kDa.
Secreted frizzled-related protein 4 (SFRP4) is a soluble Wnt-binding protein that modulates Wnt signaling. It affects bone formation and remodeling, and loss of SFRP4 causes Pyle disease, a skeletal disorder; it may also influence uterine function and apoptosis in the ovary.
| Target | Secreted frizzled-related protein 4 (gene SFRP4; UniProt Q6FHJ7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 39.8 kDa |
| Observed MW | 49 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): A549 cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human endometrial carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human SFRP4 fragment (Ala22–Lys303), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.8% and 96.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes secreted frizzled-related protein 4 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in mesenchymal cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
