| Field | Specification |
|---|---|
| Target | |
| Alternative names | SH2 domain-containing protein 3C; Cas/HEF1-associated signal transducer; Chat-H; Novel SH2-containing protein 3; SH2 domain-containing Eph receptor-binding protein 1; SHEP1; SH2D3C; NSP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SH2D3C recombinant protein (Position: D48-H789). Human SH2D3C shares 86.1% amino acid (aa) sequence identity with mouse SH2D3C. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets SH2 domain-containing protein 3C (gene SH2D3C) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 94.4 kDa, and the supplier reports an observed band at about 94 kDa.
SH2D3C (also called SHEP1 or CHAT) is an adapter protein of the NSP family that binds the scaffold BCAR1/p130Cas. Through CRK and RAPGEF1 it activates the small GTPase RAP1, supporting integrin-mediated adhesion and cell migration, and it is required for normal development of marginal zone B cells and for immune function.
| Target | SH2 domain-containing protein 3C (gene SH2D3C; UniProt Q8N5H7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 94.4 kDa |
| Observed MW | 94 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
Recombinant human SH2D3C fragment (Asp48–His789), expressed in E. coli. Sequence identity with the mouse ortholog: 86.1%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places SH2 domain-containing protein 3C in the cytoplasm, cell membrane and cell projection. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
