| Field | Specification |
|---|---|
| Target | |
| Alternative names | Testicular spindle-associated protein SHCBP1L; SHC SH2 domain-binding protein 1-like protein; SHCBP1L; C1orf14 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SHCBP1L recombinant protein (Position: K171-E506). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects testicular spindle-associated protein SHCBP1L in human and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 72.6 kDa, and the supplier reports an observed band at about 90 kDa.
SHCBP1L is a testis-specific protein that associates with the spindle in male germ cells. Working with the chaperone HSPA2, it helps maintain spindle integrity during meiosis and supports normal spermatogenesis.
| Target | Testicular spindle-associated protein SHCBP1L (gene SHCBP1L; UniProt Q9BZQ2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 72.6 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human SHCBP1L fragment (Lys171–Glu506), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places testicular spindle-associated protein SHCBP1L in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in spermatocytes and elongating spermatids inside the seminiferous tubules (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
