| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Placenta-specific protein 9; PLAC9 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human SHISA7 recombinant protein (Position: Y308-E535). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SHISA7 Antibody Picoband® is an antibody for SHISA7 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SHISA7 (placenta specific 9); UniProt: A6NL88
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 70 kDa, calculated 24145 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-SHISA7 Antibody Picoband® catalog # A18878-1.
Biological background
Biological context: Involved in cellular response to oxidative stress.
Expression and localization notes: cellular localization: Secreted., tissue context: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon..
Common research applications
- Western blotting (WB): Compare SHISA7 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SHISA7 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SHISA7-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Shisa7, a single-passing transmembrane protein, localizes at GABAergic inhibitory synapses and interacts with GABA-A receptors. Shisa7 controlled receptor abundance at synapses and sped up the channel deactivation kinetics. Shisa7 also potently enhanced the action of diazepam, a classic benzodiazepine, on GABA-A receptors. Genetic deletion of Shisa7 selectively impaired GABAergic transmission and diminished the effects of diazepam in mic
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Tissue details: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
- Research category: Cell Biology,Cell Type Markers,Tags & Cell Markers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
