| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein phosphatase non-receptor type 11;3.1.3.48;Protein-tyrosine phosphatase 1D;PTP-1D;Protein-tyrosine phosphatase 2C;PTP-2C;SH-PTP2;SHP-2;Shp2;SH-PTP3;PTPN11;PTP2C, SHPTP2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SHP2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GCA-16 is a rabbit monoclonal antibody that recognizes tyrosine-protein phosphatase non-receptor type 11 (gene PTPN11) in human samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 68.0 kDa, and the supplier reports an observed band at about 70 kDa.
SHP2 (PTPN11) is a cytoplasmic tyrosine phosphatase with two SH2 domains that, unusually, promotes signaling downstream of receptor tyrosine kinases and cytokine receptors, especially activation of the RAS–MAPK pathway. Germline PTPN11 mutations cause Noonan syndrome, and SHP2 is an active target in cancer drug research.
| Target | Tyrosine-protein phosphatase non-receptor type 11 (gene PTPN11; UniProt Q06124, human) |
|---|---|
| Clone | GCA-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 68.0 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with confirmed staining (IHC): human heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human SHP2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein phosphatase non-receptor type 11 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed, with highest levels in heart, brain, and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
