| Field | Specification |
|---|---|
| Target | |
| Alternative names | Single-minded homolog 2; Class E basic helix-loop-helix protein 15; bHLHe15; SIM2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SIM2 recombinant protein (Position: Y337-R609). Human SIM2 shares 85.8% amino acid (aa) sequence identity with mouse SIM2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes single-minded homolog 2 (gene SIM2) in human, monkey, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 73.2 kDa, and the supplier reports an observed band at about 65 kDa.
Single-minded homolog 2 (SIM2) is a bHLH-PAS transcription factor that partners with ARNT family proteins to regulate gene expression. It is a homolog of Drosophila single-minded, a key regulator of midline central nervous system development, and because the gene lies on human chromosome 21 it has been studied in relation to Down syndrome.
| Target | Single-minded homolog 2 (gene SIM2; UniProt Q14190, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 73.2 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human PC-3 cells, human HepG2 cells, monkey COS-7 cells, rat skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human SIM2 fragment (Tyr337–Arg609), expressed in E. coli. Sequence identity with the mouse ortholog: 85.8%.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places single-minded homolog 2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
