| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1; Sirp-alpha-1; Signal-regulatory protein alpha-2; Sirp-alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-3; p84; CD antigen CD172a; SIRPA; MFR; MYD1; PTPNS1; SHPS1; SIRP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SIRP alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19S17 is a rabbit monoclonal antibody that recognizes tyrosine-protein phosphatase non-receptor type substrate 1 (gene SIRPA) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 55.0 kDa, and the supplier reports an observed band at about 85 kDa.
SIRPα (CD172a, SHPS-1) is an immunoglobulin-superfamily receptor on myeloid cells and neurons whose main ligand is CD47. CD47 binding triggers SIRPα to recruit the phosphatases SHP-1 and SHP-2, delivering the well-known 'don't eat me' signal that restrains phagocytosis, which makes the CD47–SIRPα axis a major focus in macrophage and cancer immunology.
| Target | Tyrosine-protein phosphatase non-receptor type substrate 1 (gene SIRPA; UniProt P78324, human) |
|---|---|
| Clone | 19S17 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.0 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human SIRP alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein phosphatase non-receptor type substrate 1 in the membrane. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
