| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD-dependent protein deacetylase sirtuin-2;3.5.1.-;Regulatory protein SIR2 homolog 2;SIR2-like protein 2;SIRT2;SIR2L, SIR2L2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SIRT2, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody recognizes NAD-dependent protein deacetylase sirtuin-2 (gene SIRT2) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 43.2 kDa, and the supplier reports an observed band at about 36 kDa.
Sirtuin 2 (SIRT2) is an NAD+-dependent deacetylase located mainly in the cytoplasm. Its best-known substrate is α-tubulin, but it also deacetylates histone H4 lysine 16 during mitosis and a range of other proteins, so it is studied in cell cycle control, microtubule dynamics, metabolism and neurodegeneration.
| Target | NAD-dependent protein deacetylase sirtuin-2 (gene SIRT2; UniProt Q8IXJ6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 43.2 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): rat spleen tissue, human HeLa cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human SIRT2. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NAD-dependent protein deacetylase sirtuin-2 in the nucleus, cytoplasm, midbody and chromosome. Tissue expression noted by UniProt (human): Isoform 1 is expressed in heart, liver and skeletal muscle, weakly expressed in the cortex. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Miao Jinhua et al. (2023) Sirtuin 6 is a key contributor to gender differences in acute kidney injury. Cell Death Discovery. 10.1038/s41420-023-01432-y
Jinlian Li et al. (2022) Decylubiquinone Inhibits Colorectal Cancer Growth Through Upregulating Sirtuin2. Frontiers in Pharmacology. 10.3389/fphar.2021.804265
Alimova Irina et al. (2025) SIRT2 Regulates the SMARCB1 Loss-Driven Differentiation Block in ATRT. Molecular Cancer Research. 10.1158/1541-7786.MCR-24-0926
