| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD-dependent protein deacetylase sirtuin-2;3.5.1.-;Regulatory protein SIR2 homolog 2;SIR2-like protein 2;SIRT2;SIR2L, SIR2L2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SIRT2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EBA-19 is a rabbit monoclonal antibody that recognizes NAD-dependent protein deacetylase sirtuin-2 (gene SIRT2) in human and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 43.2 kDa; the supplier reports observed bands at 36, 43 kDa.
Sirtuin 2 (SIRT2) is an NAD+-dependent deacetylase located mainly in the cytoplasm. Its best-known substrate is α-tubulin, but it also deacetylates histone H4 lysine 16 during mitosis and a range of other proteins, so it is studied in cell cycle control, microtubule dynamics, metabolism and neurodegeneration.
| Target | NAD-dependent protein deacetylase sirtuin-2 (gene SIRT2; UniProt Q8IXJ6, human) |
|---|---|
| Clone | EBA-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.2 kDa |
| Observed MW | 36, 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human SIRT2.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places NAD-dependent protein deacetylase sirtuin-2 in the nucleus, cytoplasm, midbody and chromosome. Tissue expression noted by UniProt (human): Isoform 1 is expressed in heart, liver and skeletal muscle, weakly expressed in the cortex.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
