| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD-dependent protein deacylase sirtuin-6; NAD-dependent protein deacetylase sirtuin-6; Protein mono-ADP-ribosyltransferase sirtuin-6; Regulatory protein SIR2 homolog 6; hSIRT6; SIR2-like protein 6; SIRT6; SIR2L6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SIRT6 recombinant protein (Position: D14-S355). Human SIRT6 shares 82.7% amino acid (aa) sequence identity with both mouse SIRT6. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NAD-dependent protein deacylase sirtuin-6 (gene SIRT6) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
Sirtuin 6 (SIRT6) is a chromatin-bound NAD+-dependent enzyme that deacetylates histone H3 at lysines 9 and 56 and also removes long-chain fatty acyl groups from proteins. It supports double-strand break repair and telomere maintenance and represses glycolytic and NF-κB target genes, so it is a key subject in studies of genome stability, metabolism and aging.
| Target | NAD-dependent protein deacylase sirtuin-6 (gene SIRT6; UniProt Q8N6T7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human SIRT6 fragment (Asp14–Ser355), expressed in E. coli. Sequence identity with the mouse ortholog: 82.7%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places NAD-dependent protein deacylase sirtuin-6 in the nucleus, chromosome and endoplasmic reticulum.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
