Anti-SKAP2 Antibody Picoband®

Overview

Anti-SKAP2 Antibody Picoband® is an antibody for SKAP2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SKAP2 (ELAV like RNA binding protein 2); UniProt: O75563
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 41 kDa, calculated 49655 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-SKAP2 Antibody Picoband® catalog # A06280-2.

Biological background

Biological context: Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.

Expression and localization notes: cellular localization: Cytoplasm. Nucleus. Cytoplasm., tissue context: Brain; neural-specific..

Common research applications

• Western blotting (WB): Compare SKAP2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SKAP2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify SKAP2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Src kinase-associated phosphoprotein 2 is an enzyme that in humans is encoded by the SKAP2 gene. The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm. Nucleus. Cytoplasm.
• Tissue details: Brain; neural-specific.
• Research category: DNA/RNA,Epigenetics and Nuclear Signaling,RNA Processing

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.