| Field | Specification |
|---|---|
| Target | |
| Alternative names | SLAM family member 6; Activating NK receptor; NK-T-B-antigen; NTB-A; CD antigen CD352; SLAMF6; KALI |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLAMF6 recombinant protein (Position: Q22-V332). Human SLAMF6 shares 43.1% amino acid (aa) sequence identity with mouse SLAMF6. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes SLAM family member 6 (gene SLAMF6) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 37.3 kDa, and the supplier reports an observed band at about 60 kDa.
SLAMF6 (also called NTB-A or Ly108) is a self-binding receptor of the signaling lymphocytic activation molecule (SLAM) family expressed on NK cells, T cells and B cells. Signaling through the adaptor SAP modulates cytotoxicity and lymphocyte activation, and SLAMF6 is studied in T-cell exhaustion and lymphocyte biology.
| Target | SLAM family member 6 (gene SLAMF6; UniProt Q96DU3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.3 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Daudi cells, human Ramos cells, human Jurkat cells, human MOLT-4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Jurkat cells, THP-1 cells.
Immunogen
Recombinant human SLAMF6 fragment (Gln22–Val332), expressed in E. coli. Sequence identity with the mouse ortholog: 43.1%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places SLAM family member 6 in the cell membrane. Tissue expression noted by UniProt (human): Expressed by all (resting and activated) natural killer cells (NK), T- and B-lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
