| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/bile acid cotransporter;Na (+)/bile acid cotransporter;Na (+)/taurocholate transport protein;Sodium/taurocholate cotransporting polypeptide;Solute carrier family 10 member 1;Slc10a1;Ntcp; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse SLC10A1, different from the related human sequence by eighteen amino acids, and from the related rat sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects hepatic sodium/bile acid cotransporter (gene Slc10a1) in mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 39.4 kDa, and the supplier reports an observed band at about 50 kDa.
NTCP (SLC10A1) is a sodium-dependent transporter on the basolateral membrane of hepatocytes that takes up conjugated bile acids from portal blood, a key step in enterohepatic circulation. Human NTCP is also the entry receptor for hepatitis B and hepatitis D viruses, making it a major target in viral hepatitis research.
| Target | Hepatic sodium/bile acid cotransporter (gene Slc10a1; UniProt O08705, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 39.4 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (IHC): mouse liver tissue.
Samples with confirmed staining (ICC/IF): mouse liver tissue.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Synthetic peptide from the C-terminal region of mouse SLC10A1. It differs from the human sequence at eighteen residues and from the rat sequence at three residues.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places hepatic sodium/bile acid cotransporter in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed in liver in higher amounts than isoform 2... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Yue et al. (2020) Biomimetic niches reveal the minimal cues to trigger apical lumen formation in single hepatocytes. Nature Materials. 10.1038/s41563-020-0662-3
Wei-Chung Chiou et al. (2023) Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2023.116077
