| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/bile acid cotransporter; Na (+)/bile acid cotransporter; Na (+)/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1; Slc10a1; Ntcp |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse SLC10A1/NTCP1, identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects hepatic sodium/bile acid cotransporter (gene Slc10a1) in rat samples and is validated for Western blot. The predicted molecular weight is 39.4 kDa, and the supplier reports an observed band at about 50 kDa.
NTCP (SLC10A1) is a sodium-dependent transporter on the basolateral membrane of hepatocytes that takes up conjugated bile acids from portal blood, a key step in enterohepatic circulation. Human NTCP is also the entry receptor for hepatitis B and hepatitis D viruses, making it a major target in viral hepatitis research.
| Target | Hepatic sodium/bile acid cotransporter (gene Slc10a1; UniProt O08705, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 39.4 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|
Samples with a confirmed band (WB): rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of mouse SLC10A1/NTCP1. The peptide sequence is conserved in rat.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places hepatic sodium/bile acid cotransporter in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed in liver in higher amounts than isoform 2... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
