| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Solute carrier family 12 member 1;Bumetanide-sensitive sodium- (potassium)-chloride cotransporter 2;Kidney-specific Na-K-Cl symporter;SLC12A1;NKCC2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SLC12A1 (Solute carrier family 12 member 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-SLC12A1 Antibody Picoband® catalog # PB9392. Tested in IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.
- Molecular weight context: reported MW: 150-290 kDa; calculated MW: 121450 MW
- Reactivity: Human,Monkey,Mouse,Rat
- Applications: IF, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Solute carrier family 12 member 1; Solute carrier family 12 member 1. Solute carrier family 12 (sodium/potassium/chloride transporters), member 1, also called NKCC2 is specifically found in cells of the thick ascending limb of the loop of Henle in nephrons, the basic functional units of the kidney. This gene is mapped to 15q21.1. This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. This gene plays a vital role in the regulation of ionic balance and cell volume. Functional note: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Reported localization: Membrane; Multi-pass membrane protein. Expression/tissue context: Kidney specific.
Research relevance and current trends
- Channels: Researchers commonly examine how SLC12A1 (Solute carrier family 12 member 1) relates to this theme using model systems and orthogonal readouts.
- Metabolism: Researchers commonly examine how SLC12A1 (Solute carrier family 12 member 1) relates to this theme using model systems and orthogonal readouts.
- Plasma Membrane: Researchers commonly examine how SLC12A1 (Solute carrier family 12 member 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SLC12A1 (Solute carrier family 12 member 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of SLC12A1 (Solute carrier family 12 member 1) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Family / similarity context: Contains 1 PID domain.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
