| Field | Specification |
|---|---|
| Target | |
| Alternative names | Solute carrier family 12 member 1;Bumetanide-sensitive sodium- (potassium)-chloride cotransporter 2;Kidney-specific Na-K-Cl symporter;SLC12A1;NKCC2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects solute carrier family 12 member 1 (gene SLC12A1) in human, monkey, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 121.5 kDa, and the supplier reports an observed band at about 150–290 kDa.
NKCC2 (SLC12A1) is the kidney-specific Na+–K+–2Cl− cotransporter in the apical membrane of the thick ascending limb of Henle's loop. It drives salt reabsorption that underlies urine concentration, it is the target of loop diuretics such as furosemide, and its loss causes type 1 Bartter syndrome.
| Target | Solute carrier family 12 member 1 (gene SLC12A1; UniProt Q13621, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 121.5 kDa |
| Observed MW | 150–290 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): monkey kidney tissue, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human renal cancer tissue, mouse kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): mouse kidney tissue, rat kidney tissue.
Immunogen
Synthetic peptide from the N-terminal region of human SLC12A1. It differs from the mouse sequence at two residues and from the rat sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places solute carrier family 12 member 1 in the apical cell membrane. Tissue expression noted by UniProt (human): Kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
