| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium-dependent phosphate transporter 1; Gibbon ape leukemia virus receptor 1; GLVR-1; Leukemia virus receptor 1 homolog; Phosphate transporter 1; PiT-1; Solute carrier family 20 member 1; SLC20A1; GLVR1; PIT1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC20A1 recombinant protein (Position: A79-K494). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sodium-dependent phosphate transporter 1 (gene SLC20A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 73.7 kDa, and the supplier reports an observed band at about 85 kDa.
PiT1 (SLC20A1) is a sodium-coupled inorganic phosphate transporter found in most tissues. It takes up phosphate for general cell needs, has been implicated in vascular and cartilage calcification, and is required for normal cell proliferation and development; it was first identified as the receptor for gibbon ape leukemia virus.
| Target | Sodium-dependent phosphate transporter 1 (gene SLC20A1; UniProt Q8WUM9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 73.7 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat kidney tissue, rat brain tissue, rat C6 cells, mouse kidney tissue, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human colon adenocarcinoma tissue, human larynx squamous cells carcinoma tissue, human liver cancer tissue, human lung adenocarcinoma tissue, human ovary serous carcinoma tissue, human thyroid papillary carcinoma tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human rectal cancer tissue.
Immunogen
Recombinant human SLC20A1 fragment (Ala79–Lys494), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium-dependent phosphate transporter 1 in the cell membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Zong-ping et al. (2024) Uropathogenic Escherichia coli infection: innate immune disorder, bladder damage, and Tailin Fang II. Frontiers in Cellular and Infection Microbiology. 10.3389/fcimb.2024.1322119
