| Field | Specification |
|---|---|
| Target | |
| Alternative names | Solute carrier family 22 member 2;Organic cation transporter 2;hOCT2;SLC22A2;OCT2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SLC22A2, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets solute carrier family 22 member 2 (gene SLC22A2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 62.6 kDa, and the supplier reports an observed band at about 63 kDa.
Organic cation transporter 2 (OCT2, SLC22A2) is a polyspecific uniporter located on the basolateral membrane of kidney proximal tubule cells. It carries endogenous amines and many cationic drugs, such as metformin and cisplatin, from blood into the tubule cells as the first step of renal secretion, so it matters for drug clearance and drug–drug interactions.
| Target | Solute carrier family 22 member 2 (gene SLC22A2; UniProt O15244, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 62.6 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human kidney tissue, mouse kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human SLC22A2. It differs from the mouse sequence at five residues and from the rat sequence at seven residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places solute carrier family 22 member 2 in the basolateral cell membrane, basal cell membrane and apical cell membrane. Tissue expression noted by UniProt (human): Mainly expressed in kidney, in the cortex and medulla. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Holliday Michael W. Jr. et al. (2022) Potential Mechanisms Involved in Chronic Kidney Disease of Unclear Etiology. Clinical Journal of the American Society of Nephrology. 10.2215/CJN.16831221
