| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bcl-2-binding component 3;JFY-1;p53 up-regulated modulator of apoptosis;BBC3;PUMA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human SLC22A3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SLC22A3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25S19; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, Flow (as provided in the source record). Boster Bio Anti-SLC22A3 Rabbit Monoclonal Antibody catalog # M04914. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: SLC22A3 (Bcl-2-binding component 3).
- Antibody format: Monoclonal; clone 25S19; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SLC22A3 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis. . Reported cellular localization context: Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release. Tissue expression notes (as provided): Ubiquitously expressed. .
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Apoptotic Markers,Cancer,Cell Biology,Cell Death,Intracellular,Invasion/Microenvironment,Metabolism,Metabolism Processes,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Pathways and Processes,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate SLC22A3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SLC22A3 expression by Western blot in cell or tissue lysates, Detect SLC22A3 in FFPE tissue sections by immunohistochemistry, Quantify SLC22A3-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 61-80 kDa; calculated MW: 20532 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 61-80 kDa
- Cellular localization (provided): Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release.
- Tissue details (provided): Ubiquitously expressed. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
