| Field | Specification |
|---|---|
| Target | |
| Alternative names | Solute carrier family 22 member 3; Extraneuronal monoamine transporter; EMT; Organic cation transporter 3; hOCT3; SLC22A3; EMTH; OCT3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SLC22A3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25S19 is a rabbit monoclonal antibody that recognizes solute carrier family 22 member 3 (gene SLC22A3) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 61.3 kDa, and the supplier reports an observed band at about 75 kDa.
Organic cation transporter 3 (OCT3, SLC22A3) is a polyspecific transporter of organic cations with broad tissue distribution, including the brain, heart, skeletal muscle and placenta. It is often described as an extraneuronal monoamine transporter that helps clear norepinephrine, serotonin and histamine, and it also handles cationic drugs.
| Target | Solute carrier family 22 member 3 (gene SLC22A3; UniProt O75751, human) |
|---|---|
| Clone | 25S19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 61.3 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human muscle cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human SLC22A3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places solute carrier family 22 member 3 in the cell membrane, apical cell membrane, basolateral cell membrane and mitochondrion membrane. Tissue expression noted by UniProt (human): Expressed in liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiao Yu et al. (2025) Unraveling the protective role of m6A methylation in SLC22A3 expression for breast Cancer intervention. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2025.167962
