| Field | Specification |
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| Alternative Names | Mitochondrial glutamate carrier 2, Glutamate/H(+) symporter 2 Solute carrier family 25 member 18, GC-2 |
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| Conjugate | |
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| Isotype | |
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Overview
Anti-SLC25A18 (GC2) Antibody is an antibody targeting Mitochondrial glutamate carrier 2, Glutamate/H(+) symporter 2, Solute carrier family 25 member 18, GC-2 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Mitochondrial glutamate carrier 2, Glutamate/H(+) symporter 2, Solute carrier family 25 member 18, GC-2 (also reported as Mitochondrial glutamate carrier 2, Glutamate/H(+) symporter 2 Solute carrier family 25 member 18, GC-2).
- Immunogen/epitope region: Intracellular, 1st loop..
- Homology note: Rat – identical Human – 13 out of 16 amino acid identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
The solute carrier family 25 member 18 (SLC25A18) is one of the mitochondrial glutamate carriers within the SLC25 family, also known as the mitochondrial carrier family (MCF). Alongside SLC25A22, which shares similar structural and functional properties, SLC25A18 facilitates the transport of glutamate across the mitochondrial inner membrane by functioning as a symporter, coupling glutamate transport with proton movement to maintain mitochondrial pH and ionic balance1,3. SLC25A18 is predominantly expressed in the brain, liver, and testis, with comparatively lower levels in tissues like the breast, lung, and colon2,3.
Research relevance and current trends
- Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
- Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
- Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NT118.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NT118; Negative control: BLP-NT118.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
