| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial thiamine pyrophosphate carrier; Mitochondrial thiamine pyrophosphate transporter; MTPPT; Mitochondrial uncoupling protein 1; Solute carrier family 25 member 19; SLC25A19; DNC; MUP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SLC25A19, which shares 87.5% and 81.25% amino acid (aa) sequence identity with mouse and rat SLC25A19, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitochondrial thiamine pyrophosphate carrier (gene SLC25A19) in human samples and is validated for Western blot. The predicted molecular weight is 35.5 kDa, and the supplier reports an observed band at about 41 kDa.
The mitochondrial thiamine pyrophosphate carrier (SLC25A19) imports thiamine pyrophosphate, the active form of vitamin B1, into mitochondria. This cofactor is needed by the pyruvate and 2-oxoglutarate dehydrogenase complexes, and SLC25A19 mutations cause Amish lethal microcephaly and a thiamine metabolism disorder with bilateral striatal necrosis.
| Target | Mitochondrial thiamine pyrophosphate carrier (gene SLC25A19; UniProt Q9HC21, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 35.5 kDa |
| Observed MW | 41 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HEK293 cells, human PC-3 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human SLC25A19. Sequence identity with the mouse and rat orthologs: 87.5% and 81.25%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mitochondrial thiamine pyrophosphate carrier in the mitochondrion membrane. Tissue expression noted by UniProt (human): Expressed in all tissues examined except for placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
