| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial adenyl nucleotide antiporter SLC25A23; Mitochondrial ATP-Mg/Pi carrier protein 2; Short calcium-binding mitochondrial carrier protein 3; SCaMC-3; Solute carrier family 25 member 23; SLC25A23; APC2; MCSC2; SCAMC3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC25A23 recombinant protein (Position: R16-L189). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitochondrial adenyl nucleotide antiporter SLC25A23 in human, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 52.4 kDa, and the supplier reports an observed band at about 48–54 kDa.
SLC25A23 (also called SCaMC-3) is a Ca2+-regulated mitochondrial carrier that exchanges ATP-Mg or other adenine nucleotides for phosphate. By adjusting the adenine nucleotide pool in the matrix, it helps tune mitochondrial respiration and has been linked to mitochondrial Ca2+ uptake.
| Target | Mitochondrial adenyl nucleotide antiporter SLC25A23 (gene SLC25A23; UniProt Q9BV35, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.4 kDa |
| Observed MW | 48–54 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tisue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human SLC25A23 fragment (Arg16–Leu189), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial adenyl nucleotide antiporter SLC25A23 in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Expressed at low levels in most tissues examined, with highest expression in brain, skeletal muscle and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
