| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial adenyl nucleotide antiporter SLC25A24; Mitochondrial ATP-Mg/Pi carrier protein 1; Mitochondrial Ca(2+)-dependent solute carrier protein 1; Short calcium-binding mitochondrial carrier protein 1; SCaMC-1; Solute carrier family 25 member 24; SLC25A24; APC1; MCSC1; SCAMC1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC25A24 recombinant protein (Position: M1-I293). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mitochondrial adenyl nucleotide antiporter SLC25A24 in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 53.4 kDa, and the supplier reports an observed band at about 45 kDa.
SLC25A24 (also called SCaMC-1) is a Ca2+-regulated mitochondrial carrier that exchanges ATP-Mg or other adenine nucleotides for phosphate across the inner membrane. It adjusts the size of the mitochondrial adenine nucleotide pool, helps protect cells from Ca2+- and oxidative stress-induced damage, and gain-of-function variants cause Fontaine progeroid syndrome.
| Target | Mitochondrial adenyl nucleotide antiporter SLC25A24 (gene SLC25A24; UniProt Q6NUK1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 53.4 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human placenta tissue, human A431 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human diffuse large B cells lymphoma tissue, human duodenal papilla adenocarcinoma tissue, human endometrioid adenocarcinoma tissue, human glioblastoma tissue, human larynx squamous cells carcinoma tissue, human liver cancer tissue, human lung adenocarcinoma tissue, human placenta tissue, human prostate adenocarcinoma tissue, human spleen tissue, human testicular seminoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human colon cancer tissue.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human SLC25A24 fragment (Met1–Ile293), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mitochondrial adenyl nucleotide antiporter SLC25A24 in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Expressed in all tissues tested. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
