| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial adenyl nucleotide antiporter SLC25A25; Mitochondrial ATP-Mg/Pi carrier protein 3; Mitochondrial Ca(2+)-dependent solute carrier protein 3; Short calcium-binding mitochondrial carrier protein 2; SCaMC-2; Solute carrier family 25 member 25; SLC25A25; APC3; KIAA1896; MCSC3; SCAMC2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC25A25 recombinant protein (Position: M1-R437). Human SLC25A25 shares 97.3% and 96.8% amino acid (aa) sequence identity with mouse and rat SLC25A25, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitochondrial adenyl nucleotide antiporter SLC25A25 in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 52.7 kDa, and the supplier reports an observed band at about 50–55 kDa.
SLC25A25 (also called SCaMC-2) is a Ca2+-regulated mitochondrial carrier with ATP-Mg/phosphate exchange activity. It allows Ca2+ signals to change the adenine nucleotide content of mitochondria and has been implicated in metabolic efficiency and exercise capacity in mice.
| Target | Mitochondrial adenyl nucleotide antiporter SLC25A25 (gene SLC25A25; UniProt Q6KCM7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.7 kDa |
| Observed MW | 50–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human SiHa cells, human Jurkat cells, human MCF-7 cells, rat liver tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human prostate cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human SLC25A25 fragment (Met1–Arg437), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97.3% and 96.8%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial adenyl nucleotide antiporter SLC25A25 in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
